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Sugar extracts were diluted 25 times in water and 0. The amount of sugar was analyzed at nm and determined using a glucose standard curve previously constructed as reference.

Statistical analyses Analysis of data was performed using SPSS statistical analysis software version Increasing concentrations of persimmon extract in the reaction media induced a gradual decrease in the intensity of the EPR adduct signal.

Inhibition of the adduct formation could result from two reactions: The inhibition mechanism was further investigated by the 2-DR oxidative degradation assay and by lipid peroxidation experiments. Radical scavenging activity of persimmon extract. Reaction media were buffered 10 mM phosphate, pH 7.

Reactions were initiated by Fe II addition to media. Antioxidant protection from the two extracts differed significantly, with IC50 values of approximately 0. Fruits 1 to 16 had similar protective effect on 2-DR oxidation, while fruits 17 to 20 had lower antioxidant activity than the others Fig. Thus, fruits 1 to 16 were selected to the subsequent studies. In addition, the inset to Fig. Antioxidant activity of persimmon extract against 2-DR oxidation.

A Effect of persimmon extract concentration, using fruits 3 circles and 17 diamonds on 2-DR degradation. Reaction media also contained 20 mM phosphate buffer pH 7. Absorbance at nm for control values without extracts was 0. The inset shows the effect of tannic acid on 2-DR oxidation under the same experimental conditions.

In both experiments, antioxidant protection from extracts decreased with increasing iron concentrations, more prominently in the experiments with iron-EDTA.

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Effect of chelator affinity on antioxidant activity of persimmon extract in 2-DR oxidation assays. Ascorbate was added to the media after 10 min pre-incubation of other components. Incubation time was 30 min. EDTA ratios are indicated by the number above symbols. D shows the relative protection in relation to EDTA concentration. Therefore, a much more favorable equilibrium for the removal of iron from citrate is expected.

In that regard, polyphenolic compounds from persimmon pulp Gorinstein et al. This observation does not fully support the iron-chelating hypothesis in metal-mediated ROS production.

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Differently from findings in persimmon extracts, polyphenols that present a chelating mechanism, such as tannic acid and ellagic acid, had their antioxidant effectiveness reduced when Fe III -EDTA ratio had increased from 1: Effect of pre-incubation time and 2-DR concentration on antioxidant activity of persimmon extract in 2-DR oxidation assays.

After pre-incubation 0—60 minreactions were initiated with 0. B Effect of 2-DR concentration on the antioxidant activity of persimmon extracts. Photometric solutions determined with the Wilson-Devinney method suggest that BI CVn is a contact binary with a degree of contact of The asymmetry of the light curves was interpreted by the presence of dark spots on both components, and absolute parameters were determined by combining the photometric elements with the spectroscopic solutions given by Lu.

The observed period decrease can be plausibly explained by a combination of the mass transfer from the primary to the secondary and angular momentum loss via magnetic braking. The cyclic period oscillation suggests that BI CVn is a triple system containing a tertiary component with a mass no less than 0.

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As in the cases of the other contact binaries e. In that case, can the initially detached system evolve into the present contact configuration via a combination of magnetic torques from stellar winds and a case A mass transfer? The classical explosive basaltic eruption spectrum is traditionally defined by the following end member eruption styles: The field use of high-speed cameras has enabled volcanologists to make improved quantifications and more accurate descriptions of these classical eruptions styles and to quantify previously undecipherable activity including activity on the basaltic eruption spectrum between the two defined end members.

Explosive activity in at the free surface of the Halema'uma'u lava lake at Kilauea exhibited features of both sustained Hawaiian fountaining and transient Strombolian explosivity. Most of this activity is internally triggered by the internal rise of decoupled gas bubbles from below the lake's surface, but external triggering via rock falls, was also observed.